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    Identification of Unknown Bacteria
     Laboratory Exercise

    Virtual Microbiology Classroom of Science Prof Online

    Iodine Mordant for Gram Stain
    ANAT & PHYSGENERAL BIOCELL BIOMICRO 8wkMICRO 15wkINSTRUCTORS


    ID of Unknown Bacteria Lab Materials

    SPO VIRTUAL CLASSROOMS
    ADDITIONAL LABORATORY REQUIRED READINGS

    Microscopy:
    •  with narration from Sinauer Associates.
    •  from MSU. Test you中国福利彩票下载安装αppr skill to see if you中国福利彩票下载安装αpp can do a virtual streak plate procedure that produces isolated colonies.
    • : This is an extremely useful tutorial that shows, step-by-step, the Gram-staining procedure and the appearance of Gram+ and Gram- bacterial cells.
    • : Very educational video of the staining procedure.
    •  Instructions, illustration and table indicating color of cells at each step.
    •  animation and quiz from McGraw-Hill.
    •  from Microbe Library. 
    • , a YouTube video from Dr. Kimmitt.
    • , by Douglas F. Fix. Interactive page where you中国福利彩票下载安装αpp can select an area of the body and learn which normal flora typically colonize that location.

    ID of UNKNOWN BACTERIA LABORATORY VIDEOS
    ID OF UNKNOWN BACTERIA LABORATORY IMAGES
    The images linked here directly relate to the lab exercise and are designed to help you中国福利彩票下载安装αpp review and study that lab material while outside of lab. 


    ​How to Interpret MacConkey's Agar (MAC) Specialized 
    Bacterial Growth Medium

    ​How to Interpret Mannitol Salt Agar (MSA) Specialized 
    Bacterial Growth Medium
    ​How to Interpret Blood Agar (BAP) Specialized Bacterial Growth Medium
    Microbiology Lab Videos
    SCIENCE VIDEOS
    NOTE: Students must review all material in categories highlighted with 
    red titles  PRIOR TO ATTENDING LAB in order to be prepared to participate. ​​​

    In this lab, students are exposed to several means of identifying bacteria, including Gram, Acid-fast and Endospore differential stains, as well as specialized media, streak plating, dichotomous keys and the API-20E test strip. The links on this page provide the Virtual Microbiology Classroom documents, web pages and videos useful in preparing for this lab. 

    ID of UNKNOWN BACTERIA LABORATORY POWERPNTS
    ​ID of Unknown Bacteria Part I
    • Lab #3a: How to Prepare an Isolation Streak Plate 
             [PowerPoint Show or PDF Printout]
    • Lab #3a: Prepare Bacterial Smears for Staining 
            [PowerPoint Show or PDF Printout]
    ​​
    ​ID of Unknown Bacteria Part II
    • Lab #3b: Identification of Unknown Bacteria Part II  
             [PowerPoint Show or PDF Printout]

    ​​Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. Select Print, and, when the Print screen comes up, go to the Print Handling options. Under Page Scaling, select "Multiple pages per sheet", Under Pages per sheet, select "2". That uses up less paper, placing two slides on each page.

    ID of UNKNOWN BACTERIA LABORATORY DOCUMENTS
    This laboratory topic has four associated Word documents. The Lab Exercise Instructions Part I provide a thorough explanation of streak plating and how to prepare bacterial smears for differential staining. 

    Lab Exercise Instructions Part II explain how to perform differential stains (Gram, Acid-fast & Endospore), use a dichotomous key to identify bacteria and how the API-20E is used to identify Gram-negative bacteria. 

    ​ID of Unknown Bacteria Part I

    ID of Unknown Bacteria Part II 


    Additional Lab Documents:

    Virtual Microbiology
    Classroom






    You have free access to a large collection of materials used in a college-level introductory microbiology course. The Virtual Microbiology Classroom provides a wide range of free educational resources including PowerPoint Lectures, Study Guides, Review Questions and Practice Test Questions.
    Prokaryotic Cell, Mariana Ruiz
    Microbiology Photo Image Library
    SCIENCE PHOTOS
    Page last updated: 
    12/2015
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