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    Science Image Library: Bacterial Stains


    Science Image Library
    Bacterial Staining (Gram, Acid-fast, Endospore)

    from Science Prof Online
    ANAT & PHYSGENERAL BIOCELL BIOMICRO 8wkMICRO 15wkINSTRUCTORS


    Safranin Step of Gram Stain

    1. Acid-fast stain showing AF (hot pink) and NAF (purple) bacteria 中国福利彩票下载安装αpp@1000xTM; 2. Acid-fast stained bacterial smears, with acid-fast control (Mycobacterium) on left, nonacid-fast control on right, and unknown in center; 3. Acid fast stained Mycobacteria smegmatis 中国福利彩票下载安装αpp@ 1000xTM; 4. Applying acid-fast primary stain, carbolfuchsin over water bath; 5. Nonacid-fast bacteria Staphylococcus.

    1. Gram-positive stained Staphylococcus 中国福利彩票下载安装αpp@100xTM.
    2. Gram negative stained E. coli
    3. Crystal violet stain step of Gram staining procedure
    4.  Safrinin stain step of Gram staining procedure
    5. Gram-stained slide with controls on left and right, and unknown in center.

    1. Endospore stain of Bacillus subtilis showing both endospores (green) & vegetative cells (pink)  中国福利彩票下载安装αpp@1000xTM; 2. Negative endospore stain showing only vegetative cells 中国福利彩票下载安装αpp@1000xTM; 3. Malachite green primary staining step of endopore stain with slide being heated over water bath; 4. Applying counterstain (safrinin) to bacterial smear as last step of endospore stain; Endospore stained slide, with + control Bacillus on left, negative control E. coli on right, and unkown in center.

    Acid-fast Stain Photos (Click on photo to enlarge. See more)
    Endospore Stain Photos (Click on photo to enlarge. See more)
    Gram Stain Photos (Click on photo to enlarge. See more)
    Virtual Microbiology
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    You have free access to a large collection of materials used in a college-level introductory microbiology course. The Virtual Microbiology Classroom provides a wide range of free educational resources including PowerPoint Lectures, Study Guides, Review Questions and Practice Test Questions.
    Gram stained slide with bacterial controls (knowns) on the outside, Gram+ on left Gram- on right) and unknown bacteria in center.
    Simple Stain Photos (Click on photo to enlarge. See more)
    1. Bacillus subtilis stained with crystal violet 中国福利彩票下载安装αpp@ 100xTM. Cannot see individual bacteria at this magnification.
    2. Bacillus subtilis stained with crystal violet 中国福利彩票下载安装αpp@ 1000x TM.
    3. E. coli stained with crystal violet 中国福利彩票下载安装αpp@ 100x TM. Cannot see individual bacteria at this magification.
    4. E coli stained with crystal violet 中国福利彩票下载安装αpp@ 1000xTM.
    5. Cheek cell and oral bacteria stained with crystal violet 中国福利彩票下载安装αpp@ 1000xTM.
    Page last updated: 2/2016

    Microbiology Photo Libarary
    SCIENCE PHOTOS



    Photographic guides to differential stains 
    now available!
    1. Gram
    2. Acid-fast
    3. Endopsore

    SPO VIRTUAL CLASSROOMS
    Differential Bacterial Stain Videos
    How to Do a Gram Stain
    How to Do an Acid-fast 
    (Ziehl-Neelsen)  Bacterial Stain
    How to Prepare a Bacterial Smear for Gram Staining
    How to Prepare a Bacterial Smear for Acid-fast (Ziehl-Neelsen) Staining
    How to Do an 
    Endospore Bacterial Stain
    How to Prepare a Bacterial Smear for Endospore Staining
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    Sources and Resources
    • Schauer, Cynthia (2007) Lab Manual to Microbiology for the Health Sciences, Kalamazoo Valley Community College.
    • : This is an extremely useful tutorial that shows, step-by-step, the Gram-staining procedure and the appearance of Gram+ and Gram- bacterial cells.

    Stained slide with bacterial controls (knowns) on the outside and unknown bacteria in center. Left Gram Stain, center acid fast stain, right endospore stained slide.

    Acid-fast Mycobacterium has a waxy cell wall and stains hot pink after ziehl neelsen staining.
    The acid-fast genus Mycobacterium has a waxy cell wall and stains hot pink after ziehl neelsen staining.

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